Relative fluorescence units Wikipedia. The terms relative fluorescence units RFU and RFU peak refer to measurements in electrophoresis methods, such as for DNA analysis. A relative fluorescence unit is a unit of measurement used in analysis which employs fluorescence detection. 1 Fluorescence is detected using a charged coupled device CCD array, when the labeled fragments, which are separated within a capillary by using electrophoresis, are energized by laser light and travel across the detection window. A computer program measures the results, determining the quantity or size of the fragments, at each data point, from the level of fluorescence intensity. 1 Samples which contain higher quantities of amplified DNA will have higher corresponding RFU values. 23An RFU peak is a relative maximum point along a graph of the analyzed data. The data can be normalized to DNA input or additional normalizing genes. The RFU heights can range from 0 to several thousands. DNA PCR analysiseditThe RFU measurements are used, for DNA profiling, in a real time polymerase chain reaction PCR. Two common methods for detection of products in real time PCR are 1 non specific fluorescent dyes that intercalate with any double stranded DNA, and 2 sequence specific DNA probes consisting of oligonucleotides that are labeled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary DNA target. Frequently, real time PCR is combined with reverse transcription to quantify messenger RNA and Non coding RNA in cells or tissues. RFU peak thresholdseditThe RFU peak height depends on the amount of DNA being analyzed. When the amount of DNA is very low, then it can be difficult to separate a true low level RFU peak from signal noise or other technical artifacts. 4 As a result, many forensic DNA laboratories have set minimum RFU peak height levels in scoring the analysis of alleles. 4There are no firm industry wide rules for establishing minimum RFU threshold values. 4 Each laboratory, in general, has established its own threshold levels as one aspect of its particular validation procedure. Many laboratories have established both lower and upper thresholds for data interpretation, as a window of minimum and maximum readings. 4Some threshold levels can be derived experimentally based on the equipments known signal to noise ratios, or a threshold can be defined to match published data or the manufacturer specifications. 4 The company which sells the most widely used equipment for STR typing, Applied Biosystems, Inc. ABI, has recommended a peak height minimum of 1.
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What is the Best Epoxy Coating for Your Garage Floor.Trying to figure out the best garage floor epoxy coating system for your garage as well as your budget isnt always easy.With so many epoxy products, manufacturers, and commercial installers to choose from, it can be frustrating and somewhat overwhelming if you dont know exactly what you want or how an epoxy coating system works.The easiest way to tackle this problem is to break it down into the different types of epoxy products available and explain how they are installed together as systems.You can choose to purchase and install the epoxy yourself or hire a garage floor coating contractor with their own products.Either way, our goal here is to help eliminate the confusion about epoxy and to educate you on the different options available in order to choose the best epoxy for you.There are many reputable brands of epoxy available.What it comes down to when determining the best coating for your garage floor is, what type of epoxy is it and how is it used as part of a system So please read on to learn what constitutes your basic epoxy products and systems all the way to the best that you can install for your garage floor.Best Concrete Floor Crack Repair Epoxy' title='Best Concrete Floor Crack Repair Epoxy' />Waterborne Epoxy Paint.Water based epoxy is easy to apply and has a longer pot life that reduces the worry of getting it applied as quickly as other epoxy products require.Its almost as easy to apply as paint and is why its advertised as epoxy paint.Because of this, it is the favorite type that companies like to market to the DIY crowd.Wisconsins Premier Concrete Repair Provider Kevco Inc for industrial flooring and driveway repair.Concrete, epoxy, urethane, chemical resistant flooring coatings for.Your first choice is the do it yourself epoxy paint kits by brands such as Rust.Oleum or Quikrete for example. 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They can be purchased from your local home improvement center or hardware store for anywhere from 5.These kits are designed as a single coat system with roughly 5.They roll on easy and cure to a thin coat of approximately 3 mils with a matte to semi gloss finish.Though inexpensive, this is the least durable of epoxy to apply.They are based on older formulations and are not the best value for your dollar.Their resistance to stains and chemicals are not as good as other types of garage floor epoxy and they are susceptible to hot tire lift.If you like the easier application requirements of water based epoxy, you can increase the durability and protection with a more professional multi coat kit that offers a second coat of epoxy clear as a top coat.This also doubles the coating thickness from 3 mils to 6 mils.Some brands such as The Original Color Chips offer kits such as these.They are a better value for the money with more current epoxy formulations and pricing starting around 1.High Solids Epoxy.The next step up is the commercial grade epoxy products.They have the best resistance to stains, chemicals, and abrasion.They also have high solids content with the best being 1.The 1. 00 solids single coat system is much thicker and will cure to approximately 1.In fact, a single coat of 1.High solids epoxy make for a great color base coat to a multi coat system due to their high build and are available in many choices of color options as well.Cost for these commercial grade products vary depending on the solids content and brand.Expect to pay a minimum of 1.The price goes up from there for standard colors and custom tints.For an average price of 1.Though this type of epoxy is a much better value, it is not as DIY friendly as water based epoxy.It has a much thicker viscosity and needs to be back rolled when applied.The pot life is shorter as well, requiring that it be applied to the garage floor within 3.Clear Epoxy or Polyurethane Top Coats.To improve upon the single coat 1.This is referred to as the top coat.This clear coat system adds not only durability and thickness, but is also what gives your floor that deep gloss and shine.If adding a topcoat, you also have the option of adding additional paint chips to your base coat.They can be sprinkled on sparingly or they can be applied to full refusal.Full refusal is the process of throwing on chips until they dont stick anymore.There are two reasons you may want to do a full refusal broadcast.The first is that it will give the floor some texture, increase thickness for durability, and help to produce an anti slip surface.The second reason is pure cosmetics.With the multitude of different colored chip combinations to choose from, you can give your garage floor any custom look that you like.Best Garage Floor Epoxy Coating System.The best coating that you can apply to your garage floor will be a premium multi coat epoxy system.This will consist of a primer coat, 1.Its not uncommon for such a floor to be between 3.These type of floor systems can last 1.Any premium epoxy system will involve a primer.Most primers will be anywhere from 5.There are many reasons for using a primer.The thinner consistency allows for the epoxy to seep deeper into the pores of the concrete to create a better bond.It also creates better adhesion for your basecoat and helps to prevent pinholes and bubbles which are caused from out gassing of the concrete.Though this epoxy system is the most expensive of garage flooring options, this floor coating will give you the best return for your dollar.Cost varies depending on the size of your garage.Expect a minimum cost of 8.Garage flooring contractors start around 4.Final notes. If you are going to apply an epoxy coating yourself you can create any one of the above combinations.Just remember when looking at different brands, compare both the coverage rate and solids content along with the price.If product A covers 2.B covers 3. 00 square feet per container, you are much better off going with product B if you have a 5.Do not attempt to stretch it out or you are destined to receive poor results.Another factor to consider is the supplies that come with these kits.Some brands include rollers, mixing paddles, spiked shoes, paint brushes and other supplies in their price.Lastly is checking to see if they offer technical support.If this is your first time at applying a good quality epoxy and you want to make sure all your questions are answered, a manufacturer with good customer service is worth every penny.If you are looking into hiring a contractor to install an epoxy coating for your garage floor, make sure to ask how many coats they use and what each coat does.Many times flooring contractors will substitute lessor quality epoxy or less coats in order to compete in price.So remember when choosing the best epoxy coating for your garage floor and budget is to make sure you have enough to cover your floor.Multiple coats will increase the wear and durability of the floor and always go with a higher solids content if you can afford it.The dollar cost average over time for the floor is much better if you do.And finally, if applying the floor coating yourself, choose an epoxy manufacturer with good customer service to insure a good experience and a quality floor.Related articles others have read.Concrete Exchange Home.
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